Urinary iodide determined by paired-ion reversed-phase HPLC with electrochemical detection.

نویسندگان

  • J Rendl
  • S Seybold
  • W Börner
چکیده

We propose an automated method for the routine analysis of urinary iodide, using paired-ion reversed-phase HPLC with electrochemical detection and a silver working electrode. Assay conditions include a flow rate of 1.0 mL/min and an operating potential of 0.10 V. The retention time for iodide is 5.4 min. Sample preparation can be semiautomated by use of a reduced-pressure manifold. The detection threshold (signal-to-noise ratio of 3) was 2 pmol, corresponding to 0.04 mumol/L. The within-run precision (CV) for a pooled urine sample was 3.9% at 452 nmol/L iodide. The average recovery of added iodine was 94%. For comparison with a colorimetric method, we measured 177 random (untimed) urine samples by both HPLC (y) and a Technicon AutoAnalyzer acid digestion method (x). After removal of organically bound iodine, the results for unbound urinary iodide determined by the two methods were nearly identical (r = 0.99; y = -0.03 + 1.00x; Sy/x = 0.12 mumol/L). Comparison of total urinary iodine measured by the Technicon AutoAnalyzer with unbound urinary iodide determined by HPLC also showed a high correlation (r = 0.96; y = -0.03 + 0.78x; Sy/x = 0.23 mumol/L), because iodine is excreted in urine mainly as iodide. We conclude that iodine in urine can be accurately determined by the more convenient HPLC assay.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Developed Method Application for Nitrite Ion (NO2¯ ) Analysis of Tib -186 Macrophage Like Cell Lines by Rapid Isocratic HPLC System with High Sensitive Glassy Carbon Electrochemical Detector

A rapid isocratic method of high performance liquid chromatography system (HPLC) with a glassy carbon working electrode of electrochemical detector is set up for quantitative detection of  trace amount of nitrite ion (NO2¯) in aqueous protein containing cell lysate, cell media, plasma, serum, urine and other body fluids. The solid extraction  reversed phase cartridges ...

متن کامل

DETERMINATION OF AMOXYCYLLIN AND CLAVULANIC ACID IN PHARMACEUTICAL DOSAGE FORMS BY HPLC WITH AMPEROMETRIC DETECTION

A simple and sensitive high performance liquid chromatographic method with electrochemical detection is described for the quantitative determination of the amoxycillin and clavulanic acid in pharmaceutical dosage forms. Separation of sample components occurs on a reversed phase C18 column with a mobile phase consisting of methanol-water-phosphate buffer. For the amperometric detection, the pote...

متن کامل

Determination of Iodide in Beer using Monolithic Silica Disk Packed Spin Column and Reversed-phase Liquid Chromatography/Electrochemical Detection

A method for determining the concentration of iodide in beer using high-performance liquid chromatography/electrochemical detection was developed. Simple sample pretreatment of beer was performed using a monolithic silica disk-packed spin column, with a recovery rate of 84.9%. Separation of iodide ions from other endogenous compounds was carried out using a mobile phase of 10 mM sodium phosphat...

متن کامل

Determination of 4,4'-methylenebis(2-chloroaniline) in urine by liquid chromatography with ion-paired solid-phase extraction and electrochemical detection.

This highly specific and sensitive method for measuring urinary 4,4'-methylenebis(2-chloroaniline) (MBOCA) involves liquid chromatography with electrochemical detection. Before chromatography, urine samples are prepared by ion-paired solid-phase extraction on a disposable octadecylsilica column with acidic methanol solution containing 1-heptanesulfonic acid. This enhances the specificity of the...

متن کامل

Paediatric reference values for urinary catecholamine metabolites evaluated by high performance liquid chromatography and electrochemical detection.

The majority of the published reference range data on catecholamines excretion by healthy children is incomplete and often contradictory (1). We assayed in the urines of 127 healthy children the values of the catecholamines (norepinephrine, epinephrine, dopamine) and their methylated metabolites (normetanephrine, metanephrine, 3-methoxytyramine) for the determination of paediatric reference ran...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Clinical chemistry

دوره 40 6  شماره 

صفحات  -

تاریخ انتشار 1994